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Superoxide development are detected within the bloodstream and you can BALF utilizing the CMH twist probe. Blood otherwise BALF samples were collected away from PBS- and you may Bleo-treated rats and incubated quickly which have CMH. The fresh examples were transferred to the newest PTFE tube and thumb suspended, and you will EPR study is accumulated during the 77 K. The fresh concentration of nitroxide (CM.) collected inside the blood incubated which have CMH (0.dos mM) from the 37 degree for ten minute (Shape 4A).

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